Holst Publishes in Biosensors and Biomaterials
Greg Holst, PhD candidate in Georgia Tech's Bioengineering Graduate Program, published in the journal of Biosensors and Bioelectronics. The article, entitled "Rapid, quantitative, reverse transcription PCR in a polymer microfluidic chip," describes the development of a reverse transcription, microfluidic qPCR system and it's application to DNA and RNA amplification measurements.
The ability to measure real-time gene expression patterns quickly and affordably is useful for studies in stem cell biomanufacturing that require real-time observation and control. This microfluidic qRT-PCR system developed by Holst and other members of the Forest Laboratory at Georiga Tech offers a practical approach to rapid analysis.
"It uses an IR laser to heat the PCR cocktail to the correct temperatures and real-time data can be obtained in less than 1hr using a 1μL reaction volume which significantly reduces the cost and time compared to conventional PCR equipment." Holst commented. "This makes stem cell bioreactor monitoring and transient gene analysis feasible."
Holst is currently in his third year of graduate school and working in the Precision Biosystem Laboratory (PBL) run by Craig Forest, Ph.D.. His research focus is on high-speed, low cost, stem cell phenotype sensing.